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Which is the main enzyme responsible for dna replication

Enzymes Involved in DNA Replication Prokaryote

  1. The following points highlight the seven important enzymes involved in the process of DNA replication of prokaryotes. The enzymes are: 1. DNA Polymerase 2. Primase 3. Polynucleotide Ligase 4. Endonucleases 5. Pilot Proteins 6. Helicase 7. Single-Strand Binding (SSB) Protein
  2. The prokaryotic DNA polymerase II was discovered in pol A - mutant of E.coli. Pol A is a gene responsible for the synthesis of polymerase I. Therefore, the mutant of pol A - are deficient in DNA polymerase I or Kornberg enzyme. But, in absence of DNA polymerase I, replication of DNA also takes place in such mutant type
  3. Prime Replication Enzymes in Eukaryotes. There are many enzymes involved in DNA replication due to the complex nature of the whole process. Here are the main enzymes and their functions in eukaryotic cells, during cell division. Helicase. DNA replication begins at places called origins, within the DNA molecule and the creation of replication forks
  4. The main enzyme that works in DNA replication both in case of Prokaryotes and eukaryotes is DNA Polymerase, but there is structural and functional dissimilarity in both of the enzymes. DNA polymerase, as the name depicts, is responsible for adding new nucleotides (dNTPs) to the growing end of the DNA strand

3 Main Enzymes of DNA Replications Cell Biolog

Which enzyme is responsible for proofreading replication? DNA polymerases. What enzymes are involved in DNA replication quizlet? Terms in this set (9)DNA Polymerase I. Removes RNA primers and replaces them with the appropriate nucleotides during DNA replication.DNA Polymerase III. carries out all of the continuous strand DNA synthesis.Helicase. DNA polymerase is the enzyme responsible for the synthesis of new DNA molecules from the existing DNA molecules in a process known as DNA replication/DNA polymerization. The cell requires new DNA molecules in order to undergo cell division, which produces daughter cells.It carries out DNA replication in the interphase, the resting phase of the cell cycle before the nuclear division phase A variety of enzymes are involved in the process of DNA replication. The enzyme, DNA Helicase breaks the hydrogen bonds between the bases from the 5' to 3' direction, uncoiling the DNA and separating the strands. Thus, allowing other enzymes involved in the process to access each strand of DNA

DNA Replication Enzymes - Biology Wis

Answer: 1 question The main enzyme responsible for linking nucleotides during DNA replication is 1.DNA polymerase 2.RNA polymerase 3.transfer RNA 4. Ribose - the answers to estudyassistant.co In eukaryotic cells, polymerases alpha, delta, and epsilon are the primary polymerases involved in DNA replication. Because replication proceeds in the 5' to 3' direction on the leading strand, the newly formed strand is continuous. The lagging strand begins replication by binding with multiple primers

DNA Replication Enzymes of Prokaryotes and Their Roles

  1. ation. It is an enzyme-catalysed reaction. DNA Polymerase is the main enzyme in the replication process
  2. DNA Polymerase Enzyme III. It is a polymerase that is responsible for invivo replication. DNA polymerase enzyme III is a complex holoenzyme with BM 550.000 consisting of 7 different polypeptides, and carrying 5'-3' exonuclease activity while others carry 3'-5' exonuclease activity. One or more other polypeptides bind ATP molecules
  3. Click to see full answer. Considering this, what enzyme is needed for DNA replication? DNA polymerase is the enzyme that carries in the daughter nucleotides, and DNA helicase is the one that unwinds the double helix to open the replication fork. While every replication fork consists of a leading and lagging strand, each fork is actually joined to another fork in the form of a replication bubble
  4. RNA primers are needed to begin replication because DNA polymerase is unable to do it alone. DNA transcription does not have the same problem because RNA polymerase is capable of initiating RNA synthesis
  5. g a growing chain of DNA. Other proteins are also involved for initiation of the process and copying of DNA, along with proofreading capabilities to ensure the replication process takes place accurately

What are the main enzymes in DNA replication

Enzymes involved in Genetic engineering and in central dogma

The enzyme is necessary to join Okazaki fragments which are produced after discontinuous replication. The enzyme has been isolated from E.coli. It is made up of a single polypeptide chain and has a molecular weight of 70,000. Each cell of E.coli is known to posses 2000-4000 molecules of this enzyme. DNA ligase has the following functions. 1 DNA replication proposed by watson and crick (1953) following are the enzymes which are responsible for replication or duplication of dna by which new copies of dna is produced it is a bio chemical process 1)TOPOISOMERASE-It helps in the break down of hydrogen bond and solublise the dna template(old strand) The enzyme responsible for separating the two strands of DNA in a helix so that they can be copied during DNA replication. DNA Ligase The enzyme responsible for sealing together breaks or nicks in a DNA strand. Responsible for patching together Okazaki fragments on the lagging strand during DNA replication. DNA Polymerase The enzyme responsible. After the discovery of Double helical structure of DNA by Watson and Crick, they immediately proposed the DNA replication process

DNA replication - Wikipedi

Without enzymes, DNA replication would not occur. DNA Polymerases are responsible for catalysing the bonding of nucleotides, and for replacing primers DNA replication proposed by watson and crick (1953) following are the enzymes which are responsible for replication or duplication of dna by which new copies of dna is produced it is a bio chemical process 1)TOPOISOMERASE - It helps in the break down of hydrogen bond and solublise the dna template(old strand)

Answered: Which replication enzyme(s) is/are bartleb

  1. DNA ligase is the enzyme responsible for the ligation of single-strand breaks in double-stranded DNA. Generally, it is important in DNA replication, repair, and genetic recombination. On the other hand, DNA polymerase is the main enzyme responsible for the addition of complementary bases to the growing DNA strand during DNA replication
  2. Which enzyme is responsible for building the pairs in either DNA replication or protein synthesis. Polymerase
  3. The main difference between PCR and DNA replication is that PCR is an in vitro process which synthesizes DNA, while DNA replication is the in vivo process of DNA synthesis. PCR and DNA replication are two processes responsible for DNA synthesis. The enzyme responsible for DNA synthesis in PCR is a thermophilic DNA polymerase such as Taq polymerase while the enzyme responsible for DNA.
  4. In E. Without enzymes, DNA replication would not occur. Special proteins are responsible for loading primase at the origin of replication so that leading strand DNA synthesis can commence Enzyme Function in DNA replication DNA helicase: Also known as helix destabilizing enzyme. DNA Polymerase III. replication C. The enzyme is telomerase
  5. Replication Enzymes DNA helicase - unwinds and separates double stranded DNA as it moves along the DNA. DNA primase - a type of RNA polymerase that generates RNA primers. DNA polymerases - synthesize new DNA molecules by adding nucleotides to leading and lagging DNA strands

Certain classes of drugs target the enzyme responsible for relaxing DNA supercoiling ahead of the replication bubble. By preventing this enzyme from reducing the strain, the DNA often experiences random breaks and fragments, ultimately killing the cell. Which enzyme is being targeted by this typ Click here to get an answer to your question ️ Which enzyme is responsible for creating a primer that allows the dna replication process to start Name the enzyme and state its property that is responsible for continuous and discontinuous replication of the two strands of a DNA molecule. asked Oct 21, 2018 in Biology by Afreen ( 30.7k points) molecular basis of inheritanc DNA replication begins at a specific spot on the DNA molecule called the origin of replication. At the origin, enzymes unwind the double helix making its components accessible for replication. The helix is unwound by helicase to form a pair of replication forks

DNA Polymerase, the main enzyme involved in DNA synthesis requires a template (the opposite strand of DNA), and a primer (the -OH group on the THIRD carbon (not the second) of deoxyribose). In most cases of natural DNA replication, the primer for DNA synthesis and replication is a short strand of RNA (which can be made de novo) DNA polymerase I (Pol I) is primarily a repair enzyme, although it also has a function in replication. About 400 Pol I molecules exist in a single bacterium. DNA polymerase I only makes an average of 20 phosphodiester bonds before dissociating from the template First, here is a short video that might help: Enzymes involved in DNA replication are: Helicase (unwinds the DNA double helix) Gyrase (relieves the buildup of torque during unwinding) Primase (lays down RNA primers) DNA polymerase III (main DNA synthesis enzyme) DNA polymerase I (replaces RNA primers with DNA) Ligase (fills in the gaps

The resulting DNA can be merged with the DNA genome of the host cell. The main enzyme responsible for synthesis of DNA from an RNA template is called reverse transcriptase. In the case of HIV, reverse transcriptase is responsible for synthesizing a complementary DNA strand (cDNA) to the viral RNA genome Which enzyme is responsible for checking DNA for errors, and fixing it? answer choices . DNA Polymerase. DNA Primase. DNA Helicase. RNA Polymerase. Tags: What is the LAST enzyme used in DNA replication. answer choices . Topoisomerase. Telomerase. DNA Polymerase. DNA Ligase. Tags: Question 20 . SURVEY Helen Akers Date: February 09, 2021 Artificial replication of DNA enzymes is done in the lab with a process called a polymerase chain reaction.. DNA enzymes are responsible for the cell replication process. They are different proteins that copy genetic code to produce new cells. In some instances, DNA enzymes can also be used to repair or correct DNA strands 1) DNA Polymerases-I. DNA polymerase I in prokaryotes is far from irrelevant, however.This enzyme serves as a host of Clean-up functions during replication, recombination, and repair.. These special functions are enhanced by an additional enzymatic activity of DNA polymerase I, a 5'->3' exonuclease activity Question: E.coli DNA Replication Enzymes Match Each Enzyme With Its Function In E. Coli Is The Main Replication Enzyme; It Is Responsible For The Bulk Of DNA Primase DNA Synthesis. DNA Helicase Acts On The Lagging Strand To Remove RNA Primers And DNA Pol Replace Them With DNA. DNA Gyrase DNA Ligase DNA Pol IIl DNA Pol Does Not Appear To Play A Role In Replication.

molecular genetics

The enzyme responsible for the unwinding of the double helix during the process of replication is called 2. Enzymes called catalyze the synthesis of new DNA by adding to the preexisting chain 3. What is the relationship between DNA replication and the Sphase of the cell cycle? Write answer here. 4 DNA replication uses a large number of proteins and enzymes (Table \(\PageIndex{1}\)). One of the key players is the enzyme DNA polymerase, also known as DNA pol. In bacteria, three main types of DNA polymerases are known: DNA pol I, DNA pol II, and DNA pol III • DNA pol III is the main replication enzyme responsible for the bulk DNA synthesis; it adds nucleotides one by one to the growing DNA chain. 11. Replication Fork-Leading Strand • As we know DNA is antiparallel, that means one strand runs in the 3' to 5' direction and its complementary strand runs in 5' to 3' direction

DNA Replication, also known as Semi-Conservative Replication, is the process by which DNA is essentially doubled. It is an important process that takes place within the dividing cell. In this article, we shall look briefly at the structure of DNA, at the precise steps involved in replicating DNA (initiation, elongation and termination), and the clinical consequences that can occur when. DNA replication Stage one. The DNA is unwound and unzipped. The helix structure is unwound. Special molecules break the weak hydrogen bonds between bases, which are holding the two strands together

Which enzyme is responsible for unzipping the DNA double

  1. Problem 12 Medium Difficulty. The enzyme responsible for adding new nucleotides to a growing DNA chain during DNA replication is a. helicase. b. RNA polymerase. c. DNA polymerase
  2. In prokaryotes, DNA polymerase III is the main enzyme responsible for replication. DNA polymerase I and II have a role to play in repair, removing the primer and filling the gaps. In eukaryotes, DNA polymerase is the main enzyme for replication. Other DNA polymerases are involved in the repair, proofreading and primer removal
  3. g a complex, where they both play a role in initiating replication. Primase enzyme creates and places a short RNA primer which allows Pol α to start the replication process
  4. Now that you understand the basics of DNA replication, we can add a bit of complexity.The two strands of DNA have to be temporarily separated from each other; this job is done by a special enzyme, helicase, that helps unwind and separate the DNA helices (Figure 4).Another issue is that the DNA polymerase only works in one direction along the strand (5′ to 3′), but the double-stranded DNA.
  5. It is the first protein which binds to DNA to initiate DNA replication. Dna-B proteins (Mw- 3,00,000)- It is a primosome constituent and consists of six subunits. It unwinds DNA during replication. It is responsible for the extension of open complex during replication. Rep Proteins (Mw 65,000) - It is a helicase consisting of one subunit
  6. What is the protein enzyme responsible for DNA replication? mRNA transcription? for translation
An Introduction to Molecular Biology/Replication of DNA

Best DNA Replication Flashcards Quizle

Since DNA polymerase I mutants could still grow and replicate their DNA, it was realized that this must not be the main replication enzyme (it is primarily a repair enzyme). Subsequently, DNA polymerase II and III were discovered (and more) Because of the study of the DNA replication process scientists found. That the process of DNA replication is similar in bacteria and humans. But the enzymes which are used in the replication process is different in some stage. For targeting specific protein or enzyme. We have to study this mechanism of DNA replication in different organisms In DNA replication, each strand of the original DNA serves as a template for the synthesis of a complementary strand. DNA polymerase is the primary enzyme needed for replication. In transcription, a segment of DNA serves as a template for the synthesis of an RNA sequence. RNA polymerase is the primary enzyme needed for transcription Which enzyme is most directly responsible for the main process of producing a new DNA strand of the new segment, especially the Okazaki fragments. DNA ligase removes the RNA primers from the developing copy of DNA. d. DNA polymerase I removes the enzyme primase from the The mechanism of DNA replication was investigated by.

DNA replication proteinsAt the replication fork, many replication enzymes assemble on the DNA into a complex molecular machine called the replisome. The following is a list of major DNA replication enzymes that participate in the replisome: Reversal of this scheme relaxes DNA, and this mechanism also accounts for the ability of gyrase to catenate and uncatenate DNA rings Connection for AP ® Courses. As was stated previously, DNA replication is more complex than simply unzipping the double helix and making new complementary strands. Replication i In the process of DNA replication, several different enzymes have to be involved. The general purpose of DNA replication is the creation of another molecule of DNA, and that is where the enzymes come into play - to treat the parent strand of DNA and guide it through the process of replication

DNA replication employs a large number of proteins and enzymes, each of which plays a critical role during the process. One of the key players is the enzyme DNA polymerase, also known as DNA pol, which adds nucleotides one by one to the growing DNA chain that are complementary to the template strand DNA Polymerase - The enzyme responsible for catalyzing the addition of nucleotide subunits to DNA both during and after DNA replication. Primase - The enzyme responsible for initiating synthesis of.. DNA polymerase is an enzyme that helps catalyze the polymerisation of deoxyribonucleotides into a DNA strand. DNA polymerases are best known for their feedback role in DNA replication, in which the..

Which enzyme is responsible for proofreading replication

The unwinding of DNA is followed by the binding of protein dnaB at the origin of replication of DNA and remains bound to the replication fork. This binding acts as a signal for a protein called primase an RNA polymerase which synthesizes a short complementary strand at the origin of replication. Discontinuous synthesis at the Replication fork . The elongation of the primer on the template strands is done by an enzyme known as DNA polymerase DNA polymerase I (or Pol I) is an enzyme that participates in the process of prokaryotic DNA replication. Discovered by Arthur Kornberg in 1956, it was the first known DNA polymerase (and the first known of any kind of polymerase ). It was initially characterized in E. coli and is ubiquitous in prokaryotes DNA polymerase is an enzyme used in DNA replication. It synthesizes DNA on a DNA template. Bacteria have three different DNA polymerases, i.e., DNA pol I, DNA pol II and DNA pol III; while eukaryotes have several DNA polymerase enzymes. Answer verified by Topp Q. If DNA Polymerase is responsible for synthesizing DNA on the Leading Strand, what enzyme synthesizes DNA on the Lagging Strand The need for DNA replication. DNA replication is the process by which a cell makes an identical copy of its DNA. This process is performed at the beginning of every cell division so that when the.

1 . In E. coli, which DNA polymerase is the main polymerase for genome replication? [2 . What is the direction of DNA synthesis by DNA polymerases? [3 . Which of the following is not required by DNA polymerase for in vitro synthesis of DNA? [4 . Which activity of DNA polymerase I of E. coli is responsible for proofreading the newly synthesized DNA The enzyme helicase helps the DNA to unwind and separate the DNA helix into single-stranded DNA and DNA open into Y Y-shaped structure. Replication forks are formed at each replication origin as the DNA unwinds and allow replication to occur simultaneously in hundreds to thousands of locations along each chromosome The overall dna replication process is extremely. Dna polymerase iii enzyme is responsible for dna replication in vivo. Primase (lays down rna primers). Dna replication is the process of copying the dna within our cells

DNA replication takes place at a Y-shaped structure called a replication fork. A self-correcting DNA polymerase enzyme catalyzes nucleotide polymerization in a 5ʹ-to-3ʹ direction, copying a DNA template strand with remarkable fidelity. Since the two strands of a DNA double helix are antiparallel, this 5ʹ-to-3ʹ DNA synthesis can take place. What is a DNA polymerase? It is an enzyme responsible for creating DNA molecules. It is the one in charge of assembling nucleotides; which is the DNA's building block. It plays an important role in DNA replication. It comes in a pair to form two identical strands of DNA from the original DNA molecule

What is DNA Polymerase - The Biology Blo

An enzyme called helicase unwinds the DNA by breaking the hydrogen bonds between the nitrogenous base pairs. ATP hydrolysis is required for this process because it requires energy. As the DNA opens up, Y-shaped structures called replication forks are formed ( Figure 1 ) Which is the main enzyme used for the replication of DNA in E.coli ? DNA in E.coli. Answer Save. 1 Answer. Relevance. Anonymous. 8 years ago. Is there still any doubt that white DNA derived from lemmings is responsible for their blind quest for self destruction * DNA Replication Synthesis of new DNA from parental DNA called as DNA synthesis. Replication of DNA in prokaryotes required many enzymes which are listed below:- I. DNA Polymerase enzyme This enzyme is required for the polymerization of nucleo.. DNA Replication in Prokaryotes and Eukaryotes Helicase is the enzyme responsible for unwinding the DNA, generating two single-stranded template strands ahead of the polymerase. Once opened, the unpaired strands of DNA are very sticky, in a molecular sense. The free purines and pyrimidines seek out other molecules with which to hydrogen bond Solution : DNA dependent DNA polymerase enzyme is responsible for polymerisation in DNA. <br> Characteristic features of this enzyme: These enzymes are highly efficient as they catalyze polymerisation of a large no. of nucleotides in very short time in E. coli. <br> High degree of accuracy : There is a definite region in E. coli DNA where the replication originates

What is the name of the enzyme that unzips the DNA

Various enzymes will move along the individual halves, preparing the nucleotides for replication. DNA polymerase is a critical enzyme that will then assemble the new side of the DNA molecule, matching up appropriate nitrogenous base pairs with free nucleotides from the nucleus DNA replication is the process of DNA synthesis using parent DNA strands as a template. It aims at the formation of a copy of the parent DNA molecule for the daughter cell. DNA replication begins at specific locations of replication in the cell, and it produces two identical replicas of DNA from one original DNA molecule DNA replication, unwinding enzymes called DNA helicases(def)cause the two parent DNA strands to unwind and separate from one another at the origin of replication to form two Y-shaped replication forks(def). These replication forks are the actual site of DNA copying (see Fig. 3). Helix destabilizing proteinsbind to the single-strande The DNA replication is the proces in which the cell doubles its DNA contents and the DNA is later divided among two daughter cells. When the DNA doubles it happens during the S phase of the cell cycle ( S stands for Synthesis ). This steps belon..

Protein synthesis Stock Photos, Images, & Pictures

DNA Replication Step by Step Process and Enzymes involve

DNA polymerases are divided into 7 families according to their sequence homology and 3D structure similarities. The families are: Family A - DNA replication and repair (DNA Polymerase I, γ) Family B - DNA replication and repair (DNA Polymerase II, α, δ, ε). See DNA Polymerase in Thermococcus gorgonarius DNA polymerase is the enzyme responsible for generating a newly synthesized strand of DNA during S phase/DNA replication. The number of DNA polymerases in eukaryotes is much more than in prokaryotes: 14 are known, of which, five have major roles during S phase They are known as pol α, pol β, pol γ, pol δ, and pol ε

Enzymes Involved in DNA Replication Flashcards Quizle

  1. Although the polymerase reaction that is involved in DNA replication is the same, mechanistically, as the one that we saw in the transcription of structural genes on DNA onto complementary mRNA strands, the overall process of DNA replication is much more complicated. We will look first at replication i
  2. g of replication are dynamic processes that are regulated by tissue-specific, epigenetic and developmental cues
  3. Compare the roles of the enzymes DNA polymerase and DNA ligase in the replication fork of DNA. asked Mar 14, 2020 in Biology by Kasis01 ( 49.6k points) molecular basis of inheritanc
  4. al nucleotide (s) is (are) recovered and the TP becomes covalently attached to the genome ends
  5. DNA polymerase I (or Pol I) is an enzyme that participates in the process of prokaryotic DNA replication.Discovered by Arthur Kornberg in 1956, [1] it was the first known DNA polymerase (and the first known of any kind of polymerase).It was initially characterized in E. coli and is ubiquitous in prokaryotes.In E. coli and many other bacteria, the gene that encodes Pol I is known as polA
  6. DNA replication is the process of copying a double-stranded DNA molecule. Both strands serve as templates for the reproduction of the opposite strand. The process is sometimes called semi-conservative replication because the new DNA from the original strand contains half of the original and half of the newly synthesized DNA

This is the primary enzyme that is used in DNA replication. It is responsible for the synthesis of new strands by adding nucleotides to the 3′- OH group of the primer. It has a 3′-5′ exonuclease activity hence it can also proofread the errors that may arise during DNA strand replication DNA Poly III is probably referenced a hell of a lot more because it is the main enzyme complex which is involved in prokaryotic DNA replication, so it is involved in alot more than the others. Poly I really only participates in the role of DNA replication and Poly II is involved with DNA repair Restriction enzyme, protein produced by bacteria that cleaves DNA at specific sites. In bacteria, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms. Restriction enzymes are used in the laboratory to manipulate DNA fragments. Learn about the types and uses of restriction enzymes Please note that Deoxyribonucleic Acid polymerase (enzyme responsible for DNA replication is not the only meaning of DNAP. There may be more than one definition of DNAP, so check it out on our dictionary for all meanings of DNAP one by one

DNA replication is the process by which a molecule of DNA is duplicated. When a cell divides, it must first duplicate its genome so that each daughter cell winds up with a complete set of chromosomes. DNA replication is probably one of the most amazing tricks that DNA does. If you think about it. In the bacterial cell the main enzyme complex responsible for DNA replication is the DNA polymerase III (pol III) holoenzyme that consists of three sub-complexes: The core polymerase, the β-sliding clamp and the clamp-loader complex. Each pol III holoenzyme contains two core polymerases held together by the τ-subunits of the clamp loader. The enzyme responsible for joining Okazaki fragments together during DNA replication is asked Sep 25, 2015 in Biology & Microbiology by Tinderella A. DNA polymerase DNA content (nucleotide pairs per cell) 3.9.x 10. 6. about 10. 9. DNA replication rate (nucleotides/sec/rep. fork) 850 60-90 Number of replication origins/cell 1 10. 3-10. 4. Hours for complete genome replication 0.67 8. Parameters for DNA replication The replication fork is a region where a cell's DNA double helix has been unwound and separated to create an area where DNA polymerases and the other enzymes involved can use each strand as a template to synthesize a new double helix. An enzyme called a helicase catalyzes strand separation. Once the strands are separated, a group of proteins called helper proteins prevent th

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Which enzyme is responsible for assembling the dna during

The enzyme involved in the process of replication is DNA dependent DNA polymerase. This enzyme catalyzes polymerization only in one direction, i.e., 5' → 3'. As a result on template strand with 3' → 5' the replication is continuous while on the template strand with polarity 5'→3' replication is discontinuous Errors in DNA replication are rare events in a cell due to the presence of DNA ligase enzyme which fixes mistakes in the copying process. Polymerase I is solely responsible for proofreading and fixing mistakes in the copying process, which explains why so few mistakes are made BACKGROUND Oxidative stress is responsible for generating DNA lesions and the 8-oxoguanine (8-oxoG) is the most commonly lesion found in DNA damage. When this base is incorporated during DNA replication, it could generate double-strand DNA breaks and cellular death. MutT enzyme hydrolyzes the 8-oxoG

Which of the following enzymes is responsible for

Replication Enzymes DNA Polymerase: Matches the correct nucleotide and then joins adjacent nucleotides together The main types of DNA damage that occurs are: base loss and base modification. University of Leicester responsible for removing small, non-helix distorting base lesions from the genome DNA primase and DNA polymerase. DNA primase is an enzyme responsible for replication of DNA. In fact, a type of RNA polymerase which creates an RNA primer later this RNA is removed by a 5' to 3' exonuclease. The DNA polymerase uses the RNA primer for the replication of single stranded DNA. Thank You

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